Structural Analysis of Cytochrome C Genes of Major Carp and Utility of Forensic Investigation

June 2017

Rumpi G
Anil Upadhyay
Ajit kumar Roy
Samik Acharjee
Abstract

The study deals with of various bioinformatics tools and in silico modelling and analysis of cytochrome c for primary, secondary and tertiary structure prediction and Ramachandran plot assessment to understand the degree of accuracy of predicted structure. Primary structure prediction and physiochemical characterization were performed of cytochrome c by computing theoretical isoelectric point (pI) 4.71, molecular weight 26029.22Da Da, instability index 43.09, aliphatic index 110.17, extinction co-efficient 41.035 Gravy 0.297. The secondary structure assessment of cytochrome protein using psipred in local carp reveals the presence greater percentage of (16) coil Regions and (8) Beta-Strand against (6) alpha helix regions. The structure information of cytochrome protein will help us to know the role of protein in Cirhinus mrigala and Labeo rohita and interaction of their ligands. The predicted structure can also be used for molecular docking studies for more insight into the structure.The resultant protein structure was evaluated by Rampage. From Ramachandran plot assessment, it was observed that the percentage of falling in most favoured region in cytochrome c is 93.7%. Based on the findings it could be concluded that further characterization of cytochrome oxidase protein is novel and will be important for evaluating how the regulation of this protein. Analysis of DNA or protein profile helps in establishing the match of the sample DNA profile under investigation with that of the standard one. If a match is found in the sample and standard one then on its bases it can be said that both belongs to the same person and absence of match indicated lack of similarity or relationship of sample with that of the standard one. Based on the above modern Computational and Bioinformatics tools and analysis we can address questions on forensic science like forensic examinations on defendant and prosecution positions during crime investigation and criminal court proceedings, Gender determination and Forensic entomology

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